Technology
State-of-the-art whole genome sequencing equipment
Hartwig Sequencing Services uses state-of-the-art whole genome sequencing equipment, which is operated in an ISO 17025:2017 TESTING L633 accredited setup for cancer genome analysis. With our three NovaSq6000 systems we can sequence up to 60 billion reads and 23.400 Gb at a time.
At least once a year we validate the entire genome sequencing workflow to retain high quality standards. All systems are operated by trained staff with a lot of experience in the sequencing field.
ISO 17025:2017
Accredited setup for cancer genome analyses.
“The Hartwig team is very knowledgeable about all sequencing related matters and very helpful and supportive."
Mauro Muraro, CEO — Single Cell Discoveries
Mission
The mission of Hartwig Sequencing Services is to enable experiments for researchers that need a high tech sequencing laboratory with fast turn-around-times and the highest quality standards in sequencing.
Our technology
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| NovaSeq6000 | NextSeq500 | iSeq100 | Biomek i7 | Biomek 4000 | QIAsymphony | QuantStudio 12K Flex | |
|---|---|---|---|---|---|---|---|
| Utilization | Whole Genome Sequencing Whole Transcriptome Sequencing | Targeted sequencing Small genomes Transcriptomes | Small targets QC test to validate samples | DNA Library preparation | DNA/RNA Library preparation | DNA/RNA Isolation | SNP genotyping |
| Run capacity | Up to 20 billion reads | Up to 260 million reads | Up to 8 million reads | Up to 88 DNA samples | Up to 24 DNA or RNA samples | Up to 96 samples | 26 SNPs per sample |
| Read length | Up to 2x150 base pairs | Up to 2x150 base pairs | 2x150 base pairs | ||||
| Output | 135 - 3900 Gb | 25 - 140 Gb | 0.8 - 1.6 Gb | Sequencing libraries | Sequencing libraries | gDNA and total RNA | SNP calls for 26 human WGS positions |
| Data quality | 90% >Q30 on average | 90% >Q30 on average | 90% >Q30 on average | ||||
| Technology | Patterned flow cell | Patterned flow cell | Semiconductor Sequencing | Shearing/ligation | Shearing/ligation | Magnetic bead based | OpenArray™ |
| Reagents | Standard NovaSeq Reagent Kits | Standard NextSeq Reagent Kits | Standard iSeq Reagent | TruSeq | KAPA RNA HyperPrep | Standard isolation kits | TaqMan OpenArray Custom Panel |