Technology

State-of-the-art whole genome sequencing equipment

Hartwig Sequencing Services uses state-of-the-art whole genome sequencing equipment, which is operated in an ISO 17025:2017 TESTING L633 accredited setup for cancer genome analysis. With our three NovaSq6000 systems we can sequence up to 60 billion reads and 23.400 Gb at a time.

At least once a year we validate the entire genome sequencing workflow to retain high quality standards. All systems are operated by trained staff with a lot of experience in the sequencing field.

ISO 17025:2017

Accredited setup for cancer genome analyses.

Follow this link for the scope of the accreditation.

“The Hartwig team is very knowledgeable about all sequencing related matters and very helpful and supportive."
Mauro Muraro, CEO —  Single Cell Discoveries

Mission

The mission of Hartwig Sequencing Services is to enable experiments for researchers that need a high tech sequencing laboratory with fast turn-around-times and the highest quality standards in sequencing.

Our technology

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 NovaSeq6000NextSeq500iSeq100Biomek i7Biomek 4000QIAsymphonyQuantStudio 12K Flex
UtilizationWhole Genome Sequencing

Whole Transcriptome Sequencing
Targeted sequencing

Small genomes

Transcriptomes
Small targets

QC test to validate samples
DNA Library preparationDNA/RNA Library preparationDNA/RNA IsolationSNP genotyping
Run capacityUp to 20 billion readsUp to 260 million readsUp to 8 million readsUp to 88 DNA samplesUp to 24 DNA or RNA samplesUp to 96 samples26 SNPs per sample
Read lengthUp to 2x150 base pairsUp to 2x150 base pairs2x150 base pairs
Output135 - 3900 Gb25 - 140 Gb0.8 - 1.6 GbSequencing librariesSequencing librariesgDNA and total RNASNP calls for 26 human WGS positions
Data quality90% >Q30 on
average
90% >Q30 on average90% >Q30 on average
TechnologyPatterned flow cellPatterned flow cellSemiconductor SequencingShearing/ligation
Shearing/ligationMagnetic bead basedOpenArray™
ReagentsStandard NovaSeq Reagent KitsStandard NextSeq Reagent KitsStandard iSeq ReagentTruSeqKAPA RNA HyperPrep
Standard isolation kitsTaqMan OpenArray Custom Panel